Developing a diagnostic kit for early pregnancy detection in cows is crucial for enhancing reproductive management in both the dairy and beef industries. The objective is to create a kit that is not only accurate in its results but also user-friendly and cost-effective, providing farmers and veterinarians with timely and essential information. The challenge lies in devising a reliable method for early pregnancy detection that can be easily and seamlessly integrated into everyday farm management practices. Such a kit would significantly improve breeding efficiency and overall herd productivity. Currently, the inability to detect pregnancy early can lead to inefficient breeding strategies which is impacting the economic viability of cattle farming. A well-designed diagnostic kit would enable farmers to make informed decisions about breeding and managing their herds, thereby optimizing the reproductive cycle of cows. This, in turn, could lead to increased milk production in dairy farms and better weight gain in beef cattle, enhancing the overall economic returns. However, creating this diagnostic tool involves overcoming several technical and practical challenges. It must be sensitive enough to detect pregnancy at the earliest possible stage while ensuring high levels of accuracy to avoid false positives or negatives. Additionally, it must be simple enough for farmers to use without specialized training and cost-effective to be accessible for farms of all sizes. Successfully developing and implementing this diagnostic kit will be a significant advancement in the cattle industry, contributing to more sustainable and profitable farming operations.